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| Registros recuperados: 33 | |
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| Harrang, Estelle; Bierne, Nicolas; Heurtebise, Serge; Morga, Benjamin; Lapegue, Sylvie. |
| L'huître plate européenne Ostrea edulis a connu une période faste dans l'aquaculture jusqu'à la fin des années 1960, lorsque deux parasites, Marteilia refringens (1969) et Bonamia ostreae (1979), ont successivement décimé les bancs naturels de cette espèce endémique, déjà affaiblis par la sur-exploitation de ses gisements. Historiquement présente sur toute la côte européenne, de la Mer du Nord à la Mer Méditerranée, en passant par l'Océan Atlantique, la Mer Adriatique et la Mer Noire, les bancs naturels de cette espèce sont devenus très localisés, laissant penser à des phénomènes d'adaptation locale de l'huître vis à vis de son environnement biotique et abiotique. La diversité et la structuration génétique de plusieurs populations de l'espèce, à l'échelle... |
| Tipo: Text |
Palavras-chave: Huître plate; Ostrea edulis; Génétique des populations; Sélection Génétique. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00014/12547/9417.pdf |
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| Bierne, Nicolas; Eyre Walker, A. |
| A strong negative correlation between the rate of amino-acid substitution and codon usage bias in Drosophila has been attributed to interference between positive selection at nonsynonymous sites and weak selection on codon usage. To further explore this possibility we have investigated polymorphism and divergence at three kinds of sites: synonymous, nonsynonymous and intronic in relation to codon bias in D. melanogaster and D. simulans. We confirmed that protein evolution is one of the main explicative parameters for interlocus codon bias variation (r(2) similar to 40%). However, intron or synonymous diversities, which could have been expected to be good indicators of local interference [here defined as the additional increase of drift due to selection on... |
| Tipo: Text |
Palavras-chave: Substitution rate; Selective constraints; Linkage; Hill Robertson interference; GC content; Drosophila; Diversity; Codon usage. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-4221.pdf |
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| Yonemitsu, Marisa A; Giersch, Rachael M; Polo-prieto, Maria; Hammel, Maurine; Simon, Alexis; Cremonte, Florencia; Avilés, Fernando T; Merino-véliz, Nicolás; Burioli, Erika Av; Muttray, Annette F; Sherry, James; Reinisch, Carol; Baldwin, Susan A; Goff, Stephen P; Houssin, Maryline; Arriagada, Gloria; Vázquez, Nuria; Bierne, Nicolas; Metzger, Michael J. |
| Tipo: Text |
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| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00590/70255/68304.pdf |
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| Gagnaire, Pierre-alexandre; Broquet, Thomas; Aurelle, Didier; Viard, Frederique; Souissi, Ahmed; Bonhomme, Francois; Arnaud-haond, Sophie; Bierne, Nicolas. |
| Estimating the rate of exchange of individuals among populations is a central concern to evolutionary ecology and its applications to conservation and management. For instance, the efficiency of protected areas in sustaining locally endangered populations and ecosystems depends on reserve network connectivity. The population genetics theory offers a powerful framework for estimating dispersal distances and migration rates from molecular data. In the marine realm, however, decades of molecular studies have met limited success in inferring genetic connectivity, due to the frequent lack of spatial genetic structure in species exhibiting high fecundity and dispersal capabilities. This is especially true within biogeographic regions bounded by well-known... |
| Tipo: Text |
Palavras-chave: Connectivity; Gene flow; Marine conservation; Population genomics; Population structure. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00275/38592/37120.pdf |
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| Masseret, Estelle; Bierne, Nicolas; Chevret, Pascale; Roumagnac, Marie; Bec, Béatrice; Laugier, Thierry; Collos, Yves; Vaquer, Andre. |
| Coastal lagoons are morphologically and ecologically complex ecosystems, subject to constantly changing environmental conditions generally of much greater magnitude than it is recorded in the open sea. Due to their transitional nature, they usually display a number of specific features (ie. high nutrient concentrations, light spectral quality, salinity range) which are potentially influencing strategies of adaptation of photosynthetic microorganisms. The unicellular cyanobacteria Synechococcus is a major component of marine and freshwater picophytoplankton. Its genetic diversity is well documented for these ecosystems, but knowledge about biodiversity and distribution of this genus in transitional waters remains scarce. The phylogenetic analysis of a... |
| Tipo: Text |
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| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/00011/12198/8960.pdf |
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| Bierne, Nicolas; David, Pierre; Langlade, Aime; Bonhomme, F. |
| Maintaining the integrity of differentiated genomes in marine organisms needs efficient isolation mechanisms, because planktonic larval dispersion provides contacts between taxa. Habitat specialisation is interesting in this respect, because it can both prevent interspecific crosses (each taxon reproduces in its own habitat) and eliminate hybrids (typically less fit than a parental taxon in each habitat). The contact zone between smooth-shelled mussels Mytilus edulis and M. galloprovincialis in Europe is a good example, as allozyme genotypes typical of both taxa seem to segregate into different habitats. However, allozymes may be selected directly and it is not known whether the same pattern can be extended to the whole genome. Here, we used 6 presumably... |
| Tipo: Text |
Palavras-chave: Habitat specialisation; Hybrid zone; Introgression; Mytilus edulis; Mytilus galloprovincialis. |
| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/00000/10228/7597.pdf |
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| Harrang, Estelle; Lapegue, Sylvie; Morga, Benjamin; Bierne, Nicolas. |
| Marine bivalves show among the greatest allozyme diversity ever reported in Eukaryotes, putting them historically at the heart of the neutralist-selectionist controversy on the maintenance of genetic variation. Although it is now acknowledged that this high diversity is most probably a simple consequence of a large population size, convincing support for this explanation would require a rigorous assessment of the silent nucleotide diversity in natural populations of marine bivalves, which has not yet been done. This study investigated DNA sequence polymorphism in a set of 37 nuclear loci in wild samples of the flat oyster Ostrea edulis. Silent diversity was found to be only moderate (0.7%), and there was no departure from demographic equilibrium under the... |
| Tipo: Text |
Palavras-chave: Nucleotide; Polymorphism; Marine bivalve; Deleterious; Mutations; Genetic load; Ostrea edulis. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00125/23610/21444.pdf |
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| Duranton, Maud; Allal, Francois; Fraisse, Christelle; Bierne, Nicolas; Bonhomme, Francois; Gagnaire, Pierre-alexandre. |
| Speciation is a complex process that leads to the progressive establishment of reproductive isolation barriers between diverging populations. Genome-wide comparisons between closely related species have revealed the existence of heterogeneous divergence patterns, dominated by genomic islands of increased divergence supposed to contain reproductive isolation loci. However, this divergence landscape only provides a static picture of the dynamic process of speciation, during which confounding mechanisms unrelated to speciation can interfere. Here we use haplotype-resolved whole-genome sequences to identify the mechanisms responsible for the formation of genomic islands between Atlantic and Mediterranean sea bass lineages. Local ancestry patterns show that... |
| Tipo: Text |
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| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00446/55750/57409.pdf |
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| Rohfritsch, Audrey; Bierne, Nicolas; Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie. |
| Originating from the north eastern Asia, Crassostrea gigas has been introduced and translocated, mainly for aquaculture purpose, into several European countries (from Norway to Portugal and in the Mediterranean Sea) (1). Although highly variable, the invasiveness pattern of C. gigas has been demonstrated in several countries and therefore considered as a pest or a noxious species in those areas (2 and 3). Our project aims at identifying the characteristics of such a flourishing species: can its success be explained by chance and/or global warming only or does it exhibit a more important potential of adaptation than other species? Therefore we developed a population genomics approach, known as "genome scan". It corresponds to the study of numerous loci... |
| Tipo: Text |
Palavras-chave: Pacific oyster; Invasive; AFLP; Genome scan; Crassostrea gigas. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/acte-4542.pdf |
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| Bierne, Nicolas; Tanguy, A; Faure, Michel; Faure, B; David, E; Boutet, I; Boon, E; Quere, N; Plouviez, S; Kemppainen, P; Jollivet, D; Moraga, D; Boudry, Pierre; David, P. |
| We describe a simple protocol to reduce the number of cloning reactions of nuclear DNA sequences in population genetic studies of diploid organisms. Cloning is a necessary step to obtain correct haplotypes in such organisms, and, while traditional methods are efficient at cloning together many genes of a single individual, population geneticists rather need to clone the same locus in many individuals. Our method consists of marking individual sequences during the polymerase chain reaction (PCR) using 5'-tailed primers with small polynucleotide tags. PCR products are mixed together before the cloning reaction and clones are sequenced with universal plasmid primers. The individual from which a sequence comes from is identified by the tag sequences upstream... |
| Tipo: Text |
Palavras-chave: Sequence; Population genetics; High throughput allele recognition; DNA polymorphism. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-4222.pdf |
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| Sussarellu, Rossana; Huvet, Arnaud; Lapegue, Sylvie; Quillen, Virgile; Lelong, Christophe; Cornette, Florence; Jensen, Lasse Fast; Bierne, Nicolas; Boudry, Pierre. |
| Background Originating from Northeast Asia, the Pacific oyster Crassostrea gigas has been introduced into a large number of countries for aquaculture purpose. Following introduction, the Pacific oyster has turned into an invasive species in an increasing number of coastal areas, notably recently in Northern Europe. Methods To explore potential adaptation of reproductive traits in populations with different histories, we set up a common garden experiment based on the comparison of progenies from two populations of Pacific oyster sampled in France and Denmark and their hybrids. Sex ratio, condition index and microarray gene expression in gonads, were analyzed in each progeny (n = 60). Results A female-biased sex-ratio and a higher condition index were... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Adaptation; Invasiveness; Fertility; Sex-ratio; Transcriptome; Microarray. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00287/39829/38343.pdf |
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| Rohfritsch, Audrey; Bierne, Nicolas; Boudry, Pierre; Heurtebise, Serge; Cornette, Florence; Lapegue, Sylvie. |
| Originaire du nord de l’Asie, l'huître creuse, Crassostrea gigas, a été introduite et transférée, principalement pour l’aquaculture, dans plusieurs pays européens depuis le début des années 1970. Le caractère invasif de cette espèce a été observé au cours de la dernière décennie dans plusieurs pays et elle a même été considérée comme une espèce nuisible dans certaines régions, telles que la mer de Wadden. Dans le cadre du projet HiFlo, soutenu par l’ANR, nous avons pour objectif d'identifier les caractéristiques d'une telle espèce florissante: son succès peut-il être expliqué par le hasard des opportunités (expansion de leur niche écologique) ou est-il lié à des processus évolutifs, ce qui impliquerait une adaptation génétique de l'espèce à de nouvelles... |
| Tipo: Text |
Palavras-chave: Huîtres; Crassostrea gigas; Génétique; Génétique des populations. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00014/12552/9418.pdf |
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| Pepin, Jean-francois; Benabdelmouna, Abdellah; Bierne, Nicolas; Costes, Louis; Degremont, Lionel; Garcia, Celine; Guesdon, Stephane; Lamy, Jean-baptiste; Le Moine, Olivier; Morga, Benjamin; Normand, Julien; Robert, Stephane; Saunier, Alice; Soletchnik, Patrick; Travers, Marie-agnes. |
| Depuis 2014, des épisodes de mortalité anormale affectent les cheptels mytilicoles tant adultes que juvéniles. Dès l’année 2015, une étude spécifique cofinancée par la DPMA et réalisée par l’Ifremer a été initiée, l’action de recherche MORBLEU (Mortalité des moules Bleues). Les années 2015 et 2016 ont permis de dégager des éléments de connaissance et des pistes relatifs aux facteurs biotiques et abiotiques qui semblent intervenir dans le phénomène de hausse de mortalité des moules depuis 2014. Pour l’année 2017, les trois objectifs principaux étaient de : i) poursuivre la description du phénomène de mortalité ainsi que le contexte environnemental associé en effectuant une nouvelle campagne d’études et d’observations in situ, ii) tester des hypothèses... |
| Tipo: Text |
Palavras-chave: Moules; Mytilus edulis; Mortalité; Statut cytogénétique; Anomalies génomiques; Populations; Facteurs environnementaux; Ecologie microbienne. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00503/61503/65337.pdf |
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| Simon, Alexis; Arbiol, Christine; Nielsen, Einar Eg; Couteau, Jérôme; Sussarellu, Rossana; Burgeot, Thierry; Bernard, Ismaël; Coolen, Joop W. P.; Lamy, Jean-baptiste; Robert, Stephane; Skazina, Maria; Strelkov, Petr; Queiroga, Henrique; Cancio, Ibon; Welch, John J.; Viard, Frédérique; Bierne, Nicolas. |
| Human‐mediated transport creates secondary contacts between genetically differentiated lineages, bringing new opportunities for gene exchange. When similar introductions occur in different places, they provide informally replicated experiments for studying hybridisation. We here examined 4,279 Mytilus mussels, sampled in Europe and genotyped with 77 ancestry‐informative markers. We identified a type of introduced mussels, called “dock mussels,” associated with port habitats and displaying a particular genetic signal of admixture between M. edulis and the Mediterranean lineage of M. galloprovincialis. These mussels exhibit similarities in their ancestry compositions, regardless of the local native genetic backgrounds and the distance separating colonised... |
| Tipo: Text |
Palavras-chave: Admixture; Bentho-pelagic species; Biological introductions; Clines; Ports; Secondary contact. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00594/70600/68791.pdf |
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| Rey, Carine; Darnaude, Audrey; Ferraton, Franck; Guinand, Bruno; Bonhomme, François; Bierne, Nicolas; Gagnaire, Pierre-alexandre. |
| Understanding the genetic underpinnings of fitness trade-o s across spatially variable environments remains a major challenge in evolutionary biology. In Mediterranean gilthead sea bream, first-year juveniles use various marine and brackish lagoon nursery habitats characterized by a trade-o between food availability and environmental disturbance. Phenotypic di erences among juveniles foraging in di erent habitats rapidly appear after larval settlement, but the relative role of local selection and plasticity in phenotypic variation remains unclear. Here, we combine phenotypic and genetic data to address this question. We first report correlations of opposite signs between growth and condition depending on juvenile habitat type. Then, we use single... |
| Tipo: Text |
Palavras-chave: Antagonistic pleiotropy; Habitat association; Fitness trade-off; Juvenile growth; Polygenic scores; RAD-sequencing; Spatially varying selection. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00622/73370/72560.pdf |
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| Registros recuperados: 33 | |
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